CAMK2A antibody (pThr306)

Catalog No. ABIN6254013

The Rabbit Polyclonal anti-CAMK2A antibody has been validated for WB and ELISA. It is suitable to detect CAMK2A in samples from Rat. There is 1 publication available.

Quick Overview for CAMK2A antibody (pThr306) (ABIN6254013)

Target: CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CAMK2A antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CAMK2A Antibody

Binding Specificity: pThr306

Specificity: Specific for endogenous levels of the ~50 kDa α-CaM Kinase II and the ~60 kDa β-CaM Kinase II proteins phosphorylated at Thr306. Immunolabeling is completely eliminated by treatment with λ-phosphatase. It has been reported that this antibody may recognize some level of non-phosphorylated pure recombinant protein, but in our hands (as shown in our WB image) in native tissue, the antibody reacts in a phospho-specific manner.

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: bovine, canine, chicken, human, Xenopus, Zebrafish

Characteristics: Our CaM Kinase II (Thr306) rabbit polyclonal phosphospecific primary antibody is produced in-house. It detects CaM Kinase II (Thr306) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB.

Purification: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr306 of rat CaMKII, conjugated to keyhole limpet hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

Dilution Range: WB Brain: 1:1000

Dilution Range: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

Storage Comment: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Expiry Date: 12 months

References for anti-CAMK2A antibody (ABIN6254013)

Bhattacharyya, Lee, Muratcioglu, Qiu, Nyayapati, Schulman, Groves, Kuriyan: "Flexible linkers in CaMKII control the balance between activating and inhibitory autophosphorylation." in: eLife, Vol. 9, (2020) (PubMed).

Target Details for CAMK2A

Target: CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

Alternative Name: CaM Kinase II

Background:

Synonyms: Calcium/calmodulin dependent protein kinase II alpha antibody, Calcium/calmodulin dependent protein kinase II beta antibody, Calcium/calmodulin dependent protein kinase II delta antibody, Calcium/calmodulin dependent protein kinase II gamma antibody, Calcium/calmodulin-dependent protein kinase type II subunit alpha antibody, CaM kinase II alpha antibody, CaM kinase II antibody, CaM kinase II beta antibody, CaM kinase II delta antibody, CaM kinase II gamma antibody, CaM kinase II subunit alpha antibody, CaMK-II subunit alpha antibody, CAMK2 antibody, Camk2a antibody, CAMK2B antibody, CAMK2D antibody, CAMK2G antibody, CAMKA antibody, KCC2A_HUMAN antibody

Description Logic: Our CaM Kinase II (Thr306) rabbit polyclonal phosphospecific primary antibody is produced . It detects CaM Kinase II (Thr306) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB.

Gene Name Alternatives: CAMK2A/B

Molecular Weight: 50 kDa/60 kDa

Gene ID: 25400

UniProt: P11310

Pathways: WNT Signaling, Interferon-gamma Pathway, Myometrial Relaxation and Contraction